多营养层次生态养殖模式简析

系统介绍了多营养层次生态养殖模式在淡水养殖及海水养殖中的应用与发展,对比了国内外有关该养殖模式发展方向的异同,简述了该养殖模式通过提高物质和空间利用率、改善养殖环境的原理以及在我国推广应用情况,并汇总了我国沿海省份近年来开展该养殖模式选用的品种搭配及产量产值。指出了多营养层次生态养殖模式还存在的问题,对今后发展提出了建议。     

瘤胃微生物在木质纤维素价值化利用的研究进展

瘤胃是反刍动物消化的第一个腔室，各种微生物（细菌、真菌和原生动物）相互作用将木质纤维素植物生物降解为易于代谢的化合物。瘤胃也是目前自然界公认的木质纤维素高效降解和利用的天然反应器，其真菌和细菌可分泌多种木质纤维素降解酶，在木质纤维素生产生物燃料和化学用品方面具有潜在的价值。因此，本研究在瘤胃内木质纤维降解的微生物及其降解木质纤维素相关酶的基础上，重点综述了瘤胃微生物在木质纤维素生物转化为乙醇、生物化学（有机酸）及沼气等方面的研究进展，旨在为瘤胃微生物和瘤胃酶在木质纤维素价值化利用方面的研究和应用提供新的方法和思路。     

MicroRNA-145 inhibits epithelial-mesenchymal transition in human renal cancer A-498 cells by ADAM28

AIM: To investigate the inhibitory effect of microRNA-145 (miR-145) on epithelial-mesenchymal transition (EMT) in renal cancer A-498 cells. METHODS: The A-498 cells were transfected with miR-145 mimics (M145) and mimic negative control(MNC), which served as M145 group and MNC group, respectively. Mock control (MC) group was set up using untreated A-498 cells. The expression level of miR-145 in each group was detected by RT-qPCR. Transwell assay was used to detect the invasion ability of the cells. The protein expression of vimentin, E-cadherin and ADAM28 was determined by Western blot. Bioinformatic method was used to predict the target genes of miR-145. Antagonistic effect of ADAM28 over-expression on the inhibition of EMT by miR-145 was detected by Western blot. The relationship between miR-145 and ADAM28 was analyzed by dual-luciferase reporter assay. RESULTS: The expression level of miR-145 in M145 group was significantly up-regulated than that in MC group (P<0.05). The number of invasive cells in M145 group was 12.78±3.37, which was significantly lower than that in MC group (P<0.05). ADAM28 may be the target gene of miR-145. Compared with MC group, the protein expression of vimentin and ADAM28 in M145 group was significantly decreased (P<0.05), while the protein expression of E-cadherin was significantly increased (P<0.05).After ADAM28 over-expression, the protein expression of vimentin in the A-498 cells of M145 group was significantly increased (P<0.05), and the protein expression of E-cadherin was significantly decreased (P<0.05). The results of dual-lucife-irasei reporter assay showed that ADAM28 was a downstream target gene of miR-145. CONCLUSION: miR-145 may inhibit the expression of EMT-related proteins through the downstream target gene ADAM28 and inhibit the EMT process of renal cancer A-498 cells.     

QTL-allele matrix detected from RTM-GWAS is a powerful tool for studies in genetics,evolution, and breeding by design of crops

正The plant germplasm resources harboring abundant genetic variations are necessary wealth in developing new cultivars adapted to various geographic and seasonal conditions. Unraveling the complex genetic architecture underlying phenotypic diversity in germplasm population is essential     

鸡脑脊髓炎高免卵黄抗体的制备及治疗试验

鸡脑脊髓炎是由鸡脑脊髓炎病毒引起的主要侵害雏鸡的一种传染病 ,其主要特征是共济失调 ,头颈部震颤和非化脓性脑炎 ,近年来河南许多鸡场有本病的发生 ,为找到本病的有效治疗措施 ,我们开展了此项研究 ,报告如下。1　材料与方法1 .1　试验鸡　选择健康蛋用鸡 50只 ,经检查没有白痢等垂直传播的疾病 ,由郑州牧专微生物教研室饲养。1 .2　疫苗　鸡 AE弱毒苗 ,购自河南兽医防治站 ,鸡AE油乳剂灭活苗 ,由郑州牧专微生物教研室自制。1 .3　鸡 AE琼扩抗原及阳性血清　由郑州市畜牧工作总站提供。1 .4　试验鸡免疫　一免 ,每只鸡分别肌注 AE弱…     

耕牛血吸虫病检测方法比较试验

目的为了解几种检测耕牛血吸虫病的敏感性进行本试验。方法将经粪便毛蚴孵化法检出血吸虫病阳性的水牛(6头)，同时进行干卵聚酯薄膜环卵沉淀试验、间接血凝试验及快速诊断试纸盒检验的测试。结果以其检测结果与粪便毛蚴孵化法相比，阳性符合率分别为100％、100％和83％，3种方法间无显著差异。结论快速诊断试纸盒检测法以其操作简便且准确、快速、特异性高的特点，在动物血防普查工作中具有广阔的应用前景。     

水分管理方式对香根草生长发育的影响

对四种不同的水分管理条件下香根草生长研究发现：香根草最适宜在土壤水分饱和的湿润环境中生长,这时它的成活率最高,分蘖速度最快,植株高度生长也最快,定植后3个月,其对应的根体积、根干生物量、茎叶干生物量和根茎比也最高。     

The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.     

Effect of EGFP gene transfection on the cell cycle distribution of primary cultured human chondrocytes

AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37％ at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage.